Description
JAM2, also known as junctional adhesion molecule B isoform 1, is a type 1 transmembrane receptor belonging to the immunoglobulin superfamily. This protein is expressed prominently on high endothelial venules of lymphoid organs where it is localized to the intercellular boundaries of high endothelial cells. It can function as an adhesive ligand for the T cell line J45 and can interact with GM-CSF/IL-4-delived peripheral blood dendritic cells. Also, it plays a role in the regulation of transendothelial migration. It binds to very late activation antigen (VLA)-4, a leucocyte integrin that contributes to rolling and firm adhesion of lymphocytes to endothelial cells through binding to vascular cell adhesion molecule (VCAM)-1. Recombinant human JAM2, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques